WebAbstract. Availability of 4-thiouridine (4-thioU)-containing RNAs is the prerequisite for 4-thioU site-specific cross-linking studies. This paper presents a method for constructing such RNAs. A 5'- and a 3'-RNA are synthesized via phage RNA polymerase transcription and/or RNase H site-specific cleavage directed by 2'-O-methyl-RNA-DNA chimeras. WebFor this report, a qualitative assessment of stakeholders’ preferences and perceptions of ecosystem services was carried out in Thiou, in Yatenga province. Within the Enhancing the Value of Ecosystem Services in Pastoral Systems (EVESPS) project, we focused on the current state of ecosystem services, the threats to the sustainable use of such
Construction of 4-thiouridine site-specifically substituted RNAs for ...
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