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WebNov 1, 2024 · R-loop-associated plasmids were then isolated using S9.6 antibody (specific for hybrid structures) immunoprecipitation (IP) and used as templates for in vitro transcription with HeLa cell nuclear extracts. Note that both the use of a circular DNA template and the fact that the annealed RNA contain small 3′ end non-complementary … WebNov 18, 2024 · As predicted, R-loop zones of higher confidence levels were more often distributed at promoter regions (Figure 2E), and more likely associated with well-known R-loop-associated features, such as GC skew (Figure 2F), and G4 formation as determined by G4 ChIP-seq (Figure 2G), confirming that bona fide R-loops were increasingly enriched in … green shield bug location
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