Determination of aconitase activity
WebJan 1, 2024 · Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease. ... WebAconitase activity determined after 15 hours incubation at 5', pH 7.4. effect. Phosphate thus competed with the protein for the iron in the system, as well as prevented the added …
Determination of aconitase activity
Did you know?
WebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice (precooled to -20 °C) by centrifugation at 4,000 × g for 5 min. The cell pellet was resuspended in 900 μl of Tris-HCl (90 m m g; 4 °C), the supernatant was used ... WebFor quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features. Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay.
WebOct 14, 1997 · The aconitase activity was determined in the supernatant using two different methods. Method 1 is a coupled assay in which the formation of NADPH was … Web0.5M sorbitol at the concentration of 200mg/ml. Aconitase activity was determined by increase in absorbance at 340nm in the presence of 5mM citrate, 0.25mM NADP, 4mM MgCl 2, 10unit/ml of NADP-isocitrate dehydroge-nase (Murakami et al., 2006). 2.3. Determination of hydrogen peroxide and superoxide anion Determination of rosmarinic …
WebAug 29, 2024 · First, in-gel activity assays showed that the mitochondrial aconitase activity (m-aco) in FRDA patient-derived cells increased significantly after SS-31 treatment. ... Determination of ... WebThe aconitase activity was significantly depressed in the 2% O2 group (*P ⬍ 0.03, n ⫽ 6/group). ... Determination of the mechanism of free radical generation in damage in the presence of cyanosis: a new concept of controlled cardiac human aortic endothelial cells exposed to anoxia and reoxygenation. ...
WebNov 11, 2003 · Determination of Aconitase Protein Levels and Activity in the Tomato Genotypes Having demonstrated that leaves of the Aco-1 plants exhibited lower expression of the aconitase gene, we next turned our attention to determining the effect this had on the protein amount and activity.
WebBeef heart aconitase has been studied by low temper- ature magnetic circular dichroism (MCD) spectroscopy in the wavelength region 300 to I900 nm. Together with parallel electron paramagnetic resonance and ac- tivity measurements, these data enable correlations between Fe-S cluster-type and enzymic activity in aconitase. shark attack little bay video uncutWebDec 13, 2002 · Determination of activities of mitochondrial complexes. For complex activity measurements, samples of synaptosomes (1 mg) were incubated in 1 mL of standard medium for 20 min at 37°C with different concentrations of rotenone, antimycin, or cyanide, respectively. ... Aconitase activity was expressed as a function of the per cent … shark attack in swan riverWebThe osmotic and activity coefficients of aqueous mixtures of NaCl and MgCl/sub 2/ have been determined at 25/sup 0/C by using the isopiestic method. These measurements extend from low concentrations to the crystallization limits of the mixtures. They are critically compared to published isopiestic, direct vapor pressure, and emf data for this system. shark attack little bay redditWebAlper Sarikaya, Aconitase 4 trans mechanism for the reversible dehydration of citric acid as well. From this determination, Gawron et al. surmised that the alpha-hydrogen of these substrates does not participate in the role of aconitase mechanism as the trans mechanism is nowhere near the proximity of the changing subunit. Additionally, the authors found … pop star becomes first time momWebJan 10, 2024 · This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. The protocol is optimized for … shark attack little bay video originalWeblevels of aconitase, whereas its overexpression decreases its levels and activity [15, 25] (Fig. 1). This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. … shark attack little bay beachWebAconitase 2 ( ACO2 ) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been initially associated with infantile cerebellar-retinal degeneration combining optic atrophy, retinal degeneration, severe encephalopathy, epilepsy, and cerebellar ataxia1–3; … shark attack little bay uncensored